Diego and Baby Jaguar have to rescue a pair of red-eyed tree frogs floating down the river.
Diego and Baby Jaguar overhear two red-eyed tree frogs, who have fallen in the river and are heading for a waterfall. They head out to save the tree frogs, first jumping past the Cha-Cha Coconut Trees, then using his suction-cup sticky gloves to climb up a pyramid. Diego reaches the tree frogs and catches them just before they head over the waterfall. Diego returns them to the rainforest, where Alicia has found a new home with other red-eyed tree frogs.
Animal Sound of the Red-Eyed Tree Frogs
This episode uses the Nick Jr. Productions Puddle Logo from 2005-2008 on TV/VHS/DVD instead of the 1999-2005 Nick Jr. Productions Logo, and this is the first episode to do so. This is also the first show to use that, as all Go, Diego, Go! episodes from seasons 1-3 (2005-2008) use the 2005-2009 Nick Jr. Productions logo. As a matter of fact, none of the Go, Diego, Go! episodes use the 1999-2005 Nick Jr Productions logo at all, since the 2005-2009 logo first debuted on the Dora the Explorer episode Dora & Diego to the Rescue.
This is the first episode Baby Jaguar and Diego zipline after Alicia says "Go, Diego, Go!".
Contrary to how Diego's voice by both brothers Andres and Felipe Dieppa and Gabriel Alvarez were similar to one another, Diego's voice by Jake T. Austin sounded completely different from the preceding actors. When Diego was voiced by the previous three actors, he had a voice similar to that of a preteen. With Jake T. Austin (and all actors succeeding him) taking over, Diego now has a voice similar to that of an 8-year-old, mostly likely to reflect Diego's age.